欧美日本二区,久久精品国产精品亚洲毛片,国产91在线免费,亚洲精品偷拍影视在线观看,香蕉精品在线,虎白女粉嫩尤物福利视频,国产精品久久久久久久久动漫 ,在线观看日本污污ww网站,国产高清在线91福利,免费无码在外自慰喷水

    進一步提高血清促甲狀腺激素（TSH）檢測的敏感性
    增強化學(xué)發(fā)光酶免疫法測定血清促甲狀腺激素方法的建立及初步應(yīng)用
 通過正交試驗，并經(jīng)方差分析，獲得增強發(fā)光酶免疫檢測法的*實驗條件，將其與TSH-酶聯(lián)免疫吸附測定（ELISA）試劑盒相結(jié)合，建立TSH的增強化學(xué)發(fā)光酶免疫檢測法

To improve the sensitivity of measurement of TSH in serum.Methods　Establishment and application of a new chemiluminescence enzyme immunoassay to determine human thyrotropin in serum 　LIANG Yan，LIN Hanhua，WANG Muti. Department of Pediatrics, Tongji Hospital,Tongji Medical University

The authors developed a new enhanced chemiluminescence enzyme immunoassay （ECLIA）, which combined luminol-H2O2-HRP system enhanced by para-iodophenol（PIP） with TSH-ELISA test kit. The optimal experimental conditions of the enhanced chemiluminescence were obtained by orthogonal design and analysis of variance
材料和方法
儀器和試劑：Metrohm Herisau E350B-pH計（瑞士）。魯米諾 （Luminol,Serva） 用pH 10.3、0.05 mol/L 碳酸鹽緩沖液配制成5×10-2 mol/L儲備液放置3天后再以pH 8.2、0.1 mol/L 碳酸氫鈉-氫氧化鈉緩沖液稀釋備用。

將化學(xué)發(fā)光技術(shù)與TSH-ELISA試劑盒相結(jié)合，建立了TSH的增強化學(xué)發(fā)光酶免疫檢測法